Scapular dyskinesis (SD) indicates dysfunction of the scapular muscle activity during the arm elevation, resulting in altered scapular kinematics. This study examined whether SD alters scapular muscle activity and kinematics during swim stroke motion. Seventeen swimmers (mean age: 13 ± 1 years) were divided into SD ( n = 8) and control ( n = 9) groups. Scapular muscle activity (the upper, middle, and lower trapezius and the serratus anterior muscle) and kinematics data were collected and time-normalised (0-100%) during swim stroke motion by swim-bench on land. Scapular kinematics were calculated for upward rotation, internal rotation, posterior tilt, and arm elevation angles. To compare patterns of muscle activity and kinematics with and without SD, statistical parametric mapping unpaired t -test was used. The scapular upward rotation angle was decreased in SD compared to control in the 0-10% of the swim stroke phase ( p = 0.041, t* = 3.018), and the internal rotation angle was increased in 0-15% of the phase ( p = 0.033, t* = 2.994). Scapular posterior tilt and muscle activity showed no significant differences. These results suggested that SD altered scapular upward rotation and internal rotation at the initial phase of the swim stroke motion in adolescent swimmers and might potentially provoke a risk of subacromial impingement.