In Vitro Targeting of NL2 Peptide Bounded on Poly L-DOPA Coated Graphene Quantum Dot.
Mahdi MirzababaeiKambiz LarijaniHamid Hashemi-MoghaddamZohreh MirjafaryHamid MadanchiPublished in: Journal of fluorescence (2021)
Chemotherapy using drug delivery systems (DDS) can target cancer cells selectively and without affecting normal cells. In this paper, NL2 peptide as a tumor targeted peptide was bonded on the surface of poly 3,4-Dihydroxy-L-phenylalanine (Poly L-DOPA) graphene quantum dots (GQD), which was imprinted by Doxorubicin (DOX). The synthesized nanocomposite was characterized by Fourier-transform infrared spectroscopy (FTIR) and particle size was determined by dynamic light scattering (DLS) and Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). DOX release from synthesized nano-composite was investigated spectrophotometrically. Also, the toxicity and selectivity of NL2-GQD-NC on SK-BR-3 cell line were evaluated. FTIR and DLS experiment confirm the successful synthesis of Poly L-DOPA coated graphene quantum dots and their uniform particles. In vitro studies have shown that NL2-GQD-NC attached more to SK-BR-3 cells than NL2-free nanocomposites (GQD-NC). After attaching the cells could be imaged due to the presence of GQD particles and DOX release was accomplished in the tumor cells.
Keyphrases
- electron microscopy
- induced apoptosis
- quantum dots
- cell cycle arrest
- carbon nanotubes
- cancer therapy
- oxidative stress
- room temperature
- squamous cell carcinoma
- endoplasmic reticulum stress
- drug delivery
- signaling pathway
- gold nanoparticles
- locally advanced
- rectal cancer
- cell proliferation
- energy transfer
- structural basis
- ionic liquid
- highly efficient
- simultaneous determination