A Series of Zn(II) Terpyridine-Based Nitrate Complexes as Two-Photon Fluorescent Probe for Identifying Apoptotic and Living Cells via Subcellular Immigration.
Dandan LiuMingzhu ZhangWei DuLei HuFei LiXiaohe TianAidong WangQiong ZhangZhongping ZhangJieying WuYupeng TianPublished in: Inorganic chemistry (2018)
Two-photon active probe to label apoptotic cells plays a significant role in biological systems. However, discrimination of live/apoptotic cells at subcellular level under microscopy remains unachieved. Here, three novel Zn(II) terpyridine-based nitrate complexes (C1-C3) containing different pull/push units were designed. The structures of the ligands and their corresponding Zn(II) complexes were confirmed by single-crystal X-ray diffraction analysis. On the basis of the comprehensive comparison, C3 had a suitable two-photon absorption cross section in the near-infrared wavelength and good biocompatibility. Under two-photon confocal microscopy and transmission electron microscopy, it is found that C3 could target mitochondria in living cells but immigrate into the nucleolus during the apoptotic process. This dual-functional probe (C3) not only offers a valuable image tool but also acts as an indicator for cell mortality at subcellular level in a real-time manner.
Keyphrases
- living cells
- fluorescent probe
- cell death
- cell cycle arrest
- single molecule
- electron microscopy
- induced apoptosis
- high resolution
- anti inflammatory
- heavy metals
- nitric oxide
- oxidative stress
- type diabetes
- deep learning
- risk factors
- cell therapy
- magnetic resonance imaging
- endoplasmic reticulum stress
- cardiovascular disease
- mass spectrometry
- optical coherence tomography
- computed tomography
- crystal structure