Prototyping of a Microfluidic Modulator Chip and Its Application in Heart-Cut Strong-Cation-Exchange-Reversed-Phase Liquid Chromatography Coupled to Nanoelectrospray Mass Spectrometry for Targeted Proteomics.
Jelle De VosMagali DamsKen BroeckhovenGert DesmetBurkhard HorstkotteSebastiaan EeltinkPublished in: Analytical chemistry (2020)
A novel multilayer modulator chip offering a robust miniaturized interface for multidimensional liquid chromatography has been developed. The thermoplastic microfluidic device comprises five tailor-made functional layers, and the chip is compatible with commercially available switching-valve technology. The modulator chip allows for robust ultrahigh-pressure operation up to 65 MPa. Peak-dispersion characteristics of system peaks were assessed directly at the valve outlet by monitoring fluorescein injection profiles with laser-induced fluorescence detection. Integration of a microporous monolithic mixing entity in the microchannels significantly narrows the resulting peak profile. Proof-of-concept of the applicability of the microfluidic modulator chip is demonstrated in a heart-cut multidimensional strong-cation-exchange-reversed-phase liquid chromatography proteomics analysis workflow coupled to nanoelectrospray mass spectrometry for the target analysis of Glu-1-Fibrinopeptide B spiked in a protein digest mixture of bovine serum albumin.
Keyphrases
- liquid chromatography
- mass spectrometry
- circulating tumor cells
- high throughput
- high resolution mass spectrometry
- tandem mass spectrometry
- label free
- gas chromatography
- high performance liquid chromatography
- simultaneous determination
- capillary electrophoresis
- high resolution
- single cell
- solid phase extraction
- aortic valve
- mitral valve
- heart failure
- ionic liquid
- atrial fibrillation
- aortic stenosis
- protein protein
- binding protein
- amino acid
- real time pcr
- transcatheter aortic valve replacement
- psychometric properties