Focused Ultrasound Enabled Trans-Blood Brain Barrier Delivery of Gold Nanoclusters: Effect of Surface Charges and Quantification Using Positron Emission Tomography.
Deborah SultanDezhuang YeGyu Seong HeoXiaohui ZhangHannah LuehmannYimei YueLisa DeteringSergey KomarovSara TaylorYuan-Chuan TaiJoshua B RubinHong ChenYongjian LiuPublished in: Small (Weinheim an der Bergstrasse, Germany) (2018)
Focused ultrasound (FUS) technology is reported to enhance the delivery of 64 Cu-integrated ultrasmall gold nanoclusters (64 Cu-AuNCs) across the blood-brain barrier (BBB) as measured by positron emission tomography (PET). To better define the optimal physical properties for brain delivery, 64 Cu-AuNCs with different surface charges are synthesized and characterized. In vivo biodistribution studies are performed to compare the individual organ uptake of each type of 64 Cu-AuNCs. Quantitative PET imaging post-FUS treatment shows site-targeted brain penetration, retention, and diffusion of the negative, neutral, and positive 64 Cu-AuNCs. Autoradiography is performed to compare the intrabrain distribution of these nanoclusters. PET Imaging demonstrates the effective BBB opening and successful delivery of 64 Cu-AuNCs into the brain. Of the three 64 Cu-AuNCs investigated, the neutrally charged nanostructure performs the best and is the candidate platform for future theranostic applications in neuro-oncology.
Keyphrases
- pet imaging
- positron emission tomography
- blood brain barrier
- computed tomography
- aqueous solution
- cerebral ischemia
- metal organic framework
- pet ct
- white matter
- resting state
- physical activity
- sensitive detection
- photodynamic therapy
- high resolution
- palliative care
- mental health
- fluorescent probe
- high throughput
- cancer therapy
- drug delivery
- fluorescence imaging
- single cell