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Antigen-Directed Fabrication of a Multifunctional Nanovaccine with Ultrahigh Antigen Loading Efficiency for Tumor Photothermal-Immunotherapy.

Jinbin PanYaqiong WangCai ZhangXiaoyi WangHaoyu WangJiaojiao WangYizhong YuanXu WangXuejun ZhangChunshui YuShao-Kai SunXiu-Ping Yan
Published in: Advanced materials (Deerfield Beach, Fla.) (2018)
Current antigen-encapsulated multifunctional nanovaccines for oncotherapy suffer from limited antigen loading efficiency, low yield, tedious manufacture, and systemic toxicity. Here, an antigen-directed strategy for the fabrication of multifunctional nanovaccine with ultrahigh antigen loading efficiency in a facile way for tumor photothermal-immunotherapy is shown. As a proof of concept, a model antigen ovalbumin (OVA) is used as a natural carrier to load a representative theranostic agent indocyanine green (ICG). Mixing OVA and ICG in aqueous solution gives the simplest multifunctional nanovaccine so far. The nanovaccine owns antigen loading efficiency of 80.8%, high yield of >90%, intense near-infrared absorption and fluorescence, excellent reproducibility, good aqueous solubility and stability, and favorable biocompatibility. These merits not only guarantee sensitive labeling/tracking and efficient stimulation of dendritic cells, but also reliable imaging-guided photothermal-immunotherapy of tumors and tumor prevention. The proposed strategy provides a facile and robust method for large-scale and reproducible fabrication of multifunctional nanovaccines with ultrahigh antigen loading efficiency for tumor therapy.
Keyphrases
  • drug delivery
  • cancer therapy
  • dendritic cells
  • photodynamic therapy
  • metal organic framework
  • immune response
  • high resolution
  • stem cells
  • quantum dots
  • ionic liquid
  • single molecule
  • highly efficient