Autologous transplantation of photoactivated subcutaneous adipose tissue improves glucose homeostasis in high-fat diet-induced obese mice.
Rong LiKai LiLiping ZhangLin WangNi ChenXin DengMao LuoJianbo WuPublished in: Journal of tissue engineering and regenerative medicine (2019)
Increasing evidence indicates that normal adipose tissue transplantation improves whole-body energy metabolism and glucose homeostasis in a high-fat diet (HFD)-induced obese mouse model. Adipose tissue macrophages are associated with glucose homeostasis and insulin resistance in type 2 diabetes and obesity in humans, offering a potential target for therapeutics. However, whether transplantation of autologous adipose tissue that changes the macrophage phenotype directly contributes to systemic glucose intolerance has not been determined. We specifically developed our device, with more refined wavelengths of light to activate the macrophage phenotype in isolated subcutaneous white adipose tissue (sWAT) from host HFD mice. Autologous transplantation of photoactivated sWAT into HFD mice significantly reverses the M1 macrophage phenotype into M2, reduces the infiltration of macrophages in adipose tissues of HFD mice, and decreases the levels of proinflammatory cytokines. Strikingly, this transplantation reduced blood glucose levels and caused significant improvement in glucose tolerance, which was not shown in sham-operated or nonphotoactivated sWAT-transplanted HFD mice. Moreover, positron emission/computed tomography scans indicated higher glucose uptake in the heart but not in the liver, hindlimb muscles, or abdominal sWAT. These data suggested that the ability of photoactivation to shift Adipose tissue macrophage polarization in HFD mice caused a significant improvement in glucose homeostasis and that autologous transplantation might be a promising therapeutic option for the treatment of diabetes.
Keyphrases
- high fat diet
- adipose tissue
- high fat diet induced
- insulin resistance
- blood glucose
- glycemic control
- cell therapy
- type diabetes
- computed tomography
- metabolic syndrome
- mouse model
- bone marrow
- skeletal muscle
- blood pressure
- cardiovascular disease
- magnetic resonance imaging
- heart failure
- mesenchymal stem cells
- small molecule
- gene expression
- magnetic resonance
- positron emission tomography
- platelet rich plasma
- big data
- endothelial cells
- body mass index
- artificial intelligence
- smoking cessation
- weight gain
- human health
- atrial fibrillation