Comparison of Yersinia enterocolitica DNA Isolation from Swabs Without Pre-Enrichment on Selective Media With Isolation Preceded by Warm and Cold Enrichment.

Bacteriological methods for the identification of Yersinia enterocolitica are laborious and time-consuming. The aim of this study was to compare Y. enterocolitica DNA isolation from swabs without pre-enrichment on selective media with isolation preceded by warm and cold enrichment. The material for the study consisted of 150 rectal swabs taken from 50 clinically healthy fattening pigs. Forty-one Y. enterocolitica strains were isolated after warm enrichment and 43 after cold enrichment. The presence of ail , ystA , and yadA gene fragments was detected in all isolates. DNA isolation from swabs without pre-enrichment supported the detection of Y. enterocolitica in only nine samples. This study demonstrates that Y. enterocolitica DNA can be isolated from swabs without pre-enrichment on selective media, but this method is less sensitive than the approach where DNA isolation is preceded by warm or cold enrichment.