MicroRNA expression profiling of cerebrospinal fluid/serum exosomes in children with human herpesvirus 6-associated encephalitis/encephalopathy by high-throughput sequencing.
Yuka ToriiJun-Ichi KawadaKazuhiro HoribaToshihiko OkumuraTakako SuzukiYoshinori ItoPublished in: Journal of neurovirology (2022)
Primary human herpesvirus 6 (HHV-6) infection is sometimes accompanied by acute encephalopathy with reduced subcortical diffusion (AED) in immunocompetent children. We investigated exosomal microRNA (miRNA) expression profiles in cerebrospinal fluid (CSF) and sera of patients with HHV-6-associated AED (n = 5) and febrile seizure (FS) (n = 5) using high-throughput sequencing. A total of 176 and 663 miRNAs were identified in CSF and serum exosomes, respectively. Comparative analysis determined that some miRNAs (miR-381-3p, miR-155) were exclusively expressed in the CSF exosomes of AED but not of FS patients, suggesting their potential application as novel diagnostic biomarkers for AED.
Keyphrases
- cerebrospinal fluid
- high throughput sequencing
- mesenchymal stem cells
- endothelial cells
- stem cells
- young adults
- end stage renal disease
- induced pluripotent stem cells
- early onset
- newly diagnosed
- ejection fraction
- liver failure
- long non coding rna
- prognostic factors
- multiple sclerosis
- genome wide
- bone marrow
- gene expression
- climate change
- transcription factor
- risk assessment
- atomic force microscopy
- white matter
- intensive care unit
- extracorporeal membrane oxygenation