Generation of hydroxyl radical-activatable ratiometric near-infrared bimodal probes for early monitoring of tumor response to therapy.
Luyan WuYusuke IshigakiWenhui ZengTakashi HarimotoBaoli YinYinghan ChenShiyi LiaoYongchun LiuYidan SunXiaobo ZhangYing LiuYong LiangPengfei SunTakanori SuzukiGuosheng SongQu-Li FanDe-Ju YePublished in: Nature communications (2021)
Tumor response to radiotherapy or ferroptosis is closely related to hydroxyl radical (•OH) production. Noninvasive imaging of •OH fluctuation in tumors can allow early monitoring of response to therapy, but is challenging. Here, we report the optimization of a diene electrochromic material (1-Br-Et) as a •OH-responsive chromophore, and use it to develop a near-infrared ratiometric fluorescent and photoacoustic (FL/PA) bimodal probe for in vivo imaging of •OH. The probe displays a large FL ratio between 780 and 1113 nm (FL780/FL1113), but a small PA ratio between 755 and 905 nm (PA755/PA905). Oxidation of 1-Br-Et by •OH decreases the FL780/FL1113 while concurrently increasing the PA755/PA905, allowing the reliable monitoring of •OH production in tumors undergoing erastin-induced ferroptosis or radiotherapy.
Keyphrases
- living cells
- fluorescent probe
- quantum dots
- fluorescence imaging
- high resolution
- early stage
- cell death
- photodynamic therapy
- hydrogen peroxide
- radiation therapy
- locally advanced
- single molecule
- sensitive detection
- radiation induced
- small molecule
- energy transfer
- rectal cancer
- mesenchymal stem cells
- cell therapy
- replacement therapy