Charge detection mass spectrometry for the analysis of viruses and virus-like particles.
Lohra M MillerMartin F JarroldPublished in: Essays in biochemistry (2022)
Heterogeneity usually restricts conventional mass spectrometry to molecular weights less than around a megadalton. As a single-particle technique, charge detection mass spectrometry (CDMS) overcomes this limitation. In CDMS, the mass-to-charge (m/z) ratio and charge are measured simultaneously for individual ions, giving a direct mass measurement for each ion. Recent applications include the analysis of viruses, virus-like particles, vaccines, heavily glycosylated proteins, and gene therapy vectors.
Keyphrases
- mass spectrometry
- gene therapy
- liquid chromatography
- solar cells
- capillary electrophoresis
- gas chromatography
- high performance liquid chromatography
- high resolution
- loop mediated isothermal amplification
- real time pcr
- tandem mass spectrometry
- quantum dots
- simultaneous determination
- genetic diversity
- disease virus
- single molecule