OpenSTED: open-source dynamic intensity minimum system for stimulated emission depletion microscopy.

Stephanie A Pierce Jordan JacobelliKatherine S GivenWendy B MacklinJuliet T GopinathMark E SiemensDiego RestrepoEmily A Gibson

Published in: Neurophotonics (2024)

Our open-source DyMIN system is an inexpensive add-on to a conventional STED microscope that can reduce photobleaching. The system can significantly improve signal to noise for dynamic time-lapse STED imaging of live samples.

Keyphrases

high resolution
single molecule
air pollution
high intensity
high throughput
high speed
optical coherence tomography
label free
fluorescence imaging