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Changes in relative histone abundance and heterochromatin in αA-crystallin and αB-crystallin knock-in mutant mouse lenses.

Usha P AndleyBrittney N NaumannPaul D HamiltonStephanie L Bozeman
Published in: BMC research notes (2020)
In this study of histones in mutant lenses, we extracted histones from adult mouse lenses from cryaa-R49C and cryab-R120G mutant knock-in mice. We characterized the histones using matrix-assisted laser desorption/ionization time of flight (MALDI-TOF)-mass spectrometric analysis and gel electrophoresis and characterized the lens nucleus morphology using electron microscopy (EM). The relative abundance of histone H3 protein decreased in lenses from cryaa-R49C mutant mice and the relative abundance of histone H2 increased in these lenses. Electron microscopy of nuclei from cryaa-R49C-homozygous mutant mouse lenses revealed a pronounced alteration in the distribution of heterochromatin.
Keyphrases
  • wild type
  • electron microscopy
  • cataract surgery
  • mass spectrometry
  • antibiotic resistance genes
  • dna methylation
  • gene expression
  • wastewater treatment
  • skeletal muscle
  • hyaluronic acid
  • protein protein