Fluorescent Copper Probe Inhibiting Aβ1-16-Copper(II)-Catalyzed Intracellular Reactive Oxygen Species Production.
Gulshan R WalkeDnyanesh S RanadeShefali N RamtekeSrikanth RapoleCristina SatrianoEnrico RizzarelliGaetano A TomaselliGiuseppe Trusso SfrazzettoPrasad P KulkarniPublished in: Inorganic chemistry (2017)
A variety of fluorescent probes are proposed to monitor the intracellular copper content. So far, none of the probes have been evaluated for their potential to inhibit copper-associated intracellular oxidative stress. Herein, we studied the ability of a fluorescent copper probe, OBEP-CS1, to inhibit intracellular oxidative stress associated with an amyloid β (Aβ) peptide-copper complex. The data showed that OBEP-CS1 completely inhibits the copper-catalyzed oxidation as well as decarboxylation/deamination of Aβ1-16. Moreover, the cell imaging experiments confirmed that OBEP-CS1 can inhibit Aβ-CuII-catalyzed reactive oxygen species production in SH-SY5Y cells. We also demonstrated that Aβ1-16 peptide can bind intracellular copper and thereby exert oxidative stress.
Keyphrases
- reactive oxygen species
- living cells
- oxidative stress
- quantum dots
- oxide nanoparticles
- induced apoptosis
- small molecule
- dna damage
- fluorescent probe
- high resolution
- single molecule
- stem cells
- risk assessment
- hydrogen peroxide
- room temperature
- electronic health record
- cell cycle arrest
- endoplasmic reticulum stress
- nitric oxide
- big data
- nucleic acid
- cell proliferation