Design, Characterization, and Antibacterial Performance of MAPLE-Deposited Coatings of Magnesium Phosphate-Containing Silver Nanoparticles in Biocompatible Concentrations.
Denisa Alexandra FloreaValentina GrumezescuAlexandra Cătălina BîrcăBogdan Ştefan VasileMihaela MușatCristina ChircovMiruna Silvia StanAlexandru-Mihai GrumezescuEcaterina AndronescuMariana Carmen ChifiriucPublished in: International journal of molecular sciences (2022)
Bone disorders and traumas represent a common type of healthcare emergency affecting men and women worldwide. Since most of these diseases imply surgery, frequently complicated by exogenous or endogenous infections, there is an acute need for improving their therapeutic approaches, particularly in clinical conditions requiring orthopedic implants. Various biomaterials have been investigated in the last decades for their potential to increase bone regeneration and prevent orthopedic infections. The present study aimed to develop a series of MAPLE-deposited coatings composed of magnesium phosphate (Mg 3 (PO 4 ) 2 ) and silver nanoparticles (AgNPs) designed to ensure osteoblast proliferation and anti-infective properties simultaneously. Mg 3 (PO 4 ) 2 and AgNPs were obtained through the cooling bath reaction and chemical reduction, respectively, and then characterized through X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), and Selected Area Electron Diffraction (SAED). Subsequently, the obtained coatings were evaluated by Infrared Microscopy (IRM), Fourier-Transform Infrared Spectroscopy (FT-IR), and Scanning Electron Microscopy (SEM). Their biological properties show that the proposed composite coatings exhibit well-balanced biocompatibility and antibacterial activity, promoting osteoblasts viability and proliferation and inhibiting the adherence and growth of Staphylococcus aureus and Pseudomonas aeruginosa, two of the most important agents of orthopedic implant-associated infections.
Keyphrases
- electron microscopy
- silver nanoparticles
- bone regeneration
- healthcare
- signaling pathway
- pseudomonas aeruginosa
- staphylococcus aureus
- soft tissue
- minimally invasive
- biofilm formation
- public health
- emergency department
- liver failure
- high resolution
- respiratory failure
- bone mineral density
- single molecule
- optical coherence tomography
- atomic force microscopy
- escherichia coli
- body composition
- climate change
- drug delivery
- intensive care unit
- magnetic resonance imaging
- ionic liquid
- coronary artery disease
- high speed
- health information
- extracorporeal membrane oxygenation
- insulin resistance
- bone loss