Synthesis of a Dual-Color Fluorescent Dendrimer for Diagnosis of Cancer Metastasis in Lymph Nodes.
Chie KojimaKento NagaiPublished in: Polymers (2022)
Detection of cancer metastasis spread in lymph nodes is important in cancer diagnosis. In this study, a fluorescence imaging probe was designed for the detection of both lymph node and tumor cells using always-ON and activatable fluorescence probes with different colors. Rhodamine B (Rho), a matrix metalloproteinase-2 (MMP-2)-responsive green fluorescence probe, and a tumor-homing peptide were conjugated to a carboxy-terminal dendrimer that readily accumulates in lymph nodes. The activatable green fluorescence signal increased in the presence of MMP-2, which is secreted by tumor cells. Both the always-ON Rho signal and the activatable green fluorescence signal were observed from tumor cells, but only the weak always-ON Rho signal was from immune cells. Thus, this type of dendrimer may be useful for non-invasive imaging to diagnose cancer metastasis in lymph nodes.
Keyphrases
- lymph node
- fluorescence imaging
- papillary thyroid
- living cells
- squamous cell
- neoadjuvant chemotherapy
- single molecule
- sentinel lymph node
- photodynamic therapy
- fluorescent probe
- lymph node metastasis
- childhood cancer
- squamous cell carcinoma
- high resolution
- small molecule
- radiation therapy
- early stage
- loop mediated isothermal amplification
- cancer therapy
- sensitive detection
- locally advanced
- nucleic acid