Quantitation and mapping of the epigenetic marker 5-hydroxymethylcytosine.
Ying QingZhiqi TianYing BiYongyao WangJiangang LongChun-Xiao SongJiajie DiaoPublished in: BioEssays : news and reviews in molecular, cellular and developmental biology (2017)
We here review primary methods used in quantifying and mapping 5-hydroxymethylcytosine (5hmC), including global quantification, restriction enzyme-based detection, and methods involving DNA-enrichment strategies and the genome-wide sequencing of 5hmC. As discovered in the mammalian genome in 2009, 5hmC, oxidized from 5-methylcytosine (5mC) by ten-eleven translocation (TET) dioxygenases, is increasingly being recognized as a biomarker in biological processes from development to pathogenesis, as its various detection methods have shown. We focus in particular on an ultrasensitive single-molecule imaging technique that can detect and quantify 5hmC from trace samples and thus offer information regarding the distance-based relationship between 5hmC and 5mC when used in combination with fluorescence resonance energy transfer.
Keyphrases
- single molecule
- energy transfer
- genome wide
- high resolution
- quantum dots
- dna methylation
- label free
- atomic force microscopy
- living cells
- mass spectrometry
- loop mediated isothermal amplification
- gold nanoparticles
- gene expression
- real time pcr
- high density
- ms ms
- healthcare
- liquid chromatography tandem mass spectrometry
- risk assessment
- high performance liquid chromatography
- low density lipoprotein
- cell free
- single cell
- high speed
- sensitive detection
- photodynamic therapy