A Bio-inspired Multifunctionalized Silk Fibroin.
Sofia SantiInes ManciniSandra DirèEmanuela CalloneSperanza GiorgioNicola Maria PugnoClaudio MigliaresiAntonella MottaPublished in: ACS biomaterials science & engineering (2021)
A bio-inspired multifunctionalized silk fibroin (BMS) was synthesized in order to mimic the interaction of nidogen with the type IV collagen and laminin of basement membranes. The designed BMS consists of a motif of laminin α-chain-derived, called IK peptide, and type IV collagen covalently bound to the silk fibroin (SF) by using EDC/NHS coupling and a Cu-free click chemistry reaction, respectively. Silk fibroin was chosen as the main component of the BMS because it is versatile and biocompatible, induces an in vivo favorable bioresponse, and moreover can be functionalized with different methods. The chemical structure of BMS was analyzed by using X-ray photoelectron spectroscopy, attenuated total reflection-Fourier transform infrared, cross-polarization magic angle spinning nuclear magnetic resonance techniques, and colorimetric assay. The SF and BMS solutions were cross-linked by sonication to form hydrogels or casted to make films in order to evaluate and compare the early adhesion and viability of MRC5 cells. BMS hydrogels were also characterized by rheological and thermal analyses.
Keyphrases
- tissue engineering
- magnetic resonance
- high resolution
- wound healing
- room temperature
- induced apoptosis
- solid state
- gold nanoparticles
- drug delivery
- oxidative stress
- patient safety
- single molecule
- hydrogen peroxide
- mass spectrometry
- drug release
- living cells
- escherichia coli
- cystic fibrosis
- extracellular matrix
- contrast enhanced
- nitric oxide
- drug discovery
- quality improvement
- signaling pathway
- dual energy
- electron transfer
- hyaluronic acid