Natural Berberine-Based Chinese Herb Medicine Assembled Nanostructures with Modified Antibacterial Application.
Tong LiPenglong WangWenbo GuoXuemei HuangXuehao TianGaorong WuBing XuFeifei LiCong YanXing-Jie LiangHaimin LeiPublished in: ACS nano (2019)
The abuse of traditional antibiotics has caused a series of health problems including antimicrobial resistance, which threatens human health. Therefore, searching for broad sources of antimicrobial agents and developing multidimensional strategies to combat bacterial infections are urgent. Here, we reported two natural self-assembling modes between berberine (BBR) and flavonoid glycosides: nanoparticles (NPs) and nanofibers (NFs), which were both mainly governed by electrostatic and hydrophobic interactions. These two nanostructures exhibited different antibacterial properties from BBR. NPs showed significantly enhanced bacteriostatic activity, whereas NFs displayed a much weaker effect than BBR. The distinguishing properties can be attributed to the different spatial configurations and self-assembly processes of NPs and NFs. Flavonoid glycosides and BBR first formed a one-dimensional complex unit and subsequently self-assembled into three-dimensional nanostructures. With the hydrophilic glucuronic acid toward the outside, NPs exhibited stronger affinity to bacteria, thereby inducing the collapse of the bacteria population and the decrease in biofilm. In addition, in vitro hemolysis tests, cytotoxicity tests, and in vivo zebrafish toxicity evaluation showed that the obtained self-assemblies had good biocompatibility. This supramolecular self-assembly strategy can be applied to construct other nanoscale antibacterial drugs and thus provides weapons for the development of self-delivering drugs in bacterial infection treatment.
Keyphrases
- human health
- antimicrobial resistance
- oxide nanoparticles
- risk assessment
- silver nanoparticles
- staphylococcus aureus
- mental health
- healthcare
- climate change
- pseudomonas aeruginosa
- essential oil
- anti inflammatory
- oxidative stress
- drinking water
- wound healing
- molecular dynamics simulations
- biofilm formation
- health information
- escherichia coli
- atomic force microscopy
- combination therapy
- liquid chromatography
- cystic fibrosis
- quantum dots