Optimization of monocyte gating to quantify monocyte subsets for the diagnosis of chronic myelomonocytic leukemia.
Rebeca JuradoMaria HuguetBlanca XicoyMarta CabezonAri Jimenez-PonceDavid QuintelaCristina De La FuenteMinerva RayaEsther VinetsJordi JuncaJoaquim Julià-TorrasLurdes ZamoraAlbert OriolJose-Tomas NavarroXavier CalvoMarc SoriguePublished in: Cytometry. Part B, Clinical cytometry (2022)
The choice of monocyte gating strategy appears relevant for the monocyte subset distribution test. Our 10-color proposal allowed satisfactory monocyte gating even in highly abnormal PB. Computational analysis seems promising to increase reproducibility in monocyte subset quantification.