Optimization of monocyte gating to quantify monocyte subsets for the diagnosis of chronic myelomonocytic leukemia.

Rebeca JuradoMaria HuguetBlanca XicoyMarta CabezonAri Jimenez-PonceDavid QuintelaCristina De La FuenteMinerva RayaEsther VinetsJordi JuncaJoaquim Julià-TorrasLurdes ZamoraAlbert OriolJose-Tomas NavarroXavier CalvoMarc Sorigue

Published in: Cytometry. Part B, Clinical cytometry (2022)

The choice of monocyte gating strategy appears relevant for the monocyte subset distribution test. Our 10-color proposal allowed satisfactory monocyte gating even in highly abnormal PB. Computational analysis seems promising to increase reproducibility in monocyte subset quantification.

Keyphrases

dendritic cells
peripheral blood
endothelial cells
immune response
bone marrow
heavy metals
risk assessment
drug induced