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Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater.

Antonio RodríguezMario Vaneechoutte
Published in: BMC microbiology (2019)
In our hands, the most efficient cell lysis and highest RNA yield from C. albicans cells stored in RNAlater was obtained by horizontal bead beating in RiboPure Lysis Buffer followed by RNA extraction with the RiboPure Yeast Kit.
Keyphrases
  • candida albicans
  • single cell
  • biofilm formation
  • cell therapy
  • induced apoptosis
  • room temperature
  • nucleic acid
  • stem cells
  • signaling pathway
  • mesenchymal stem cells
  • oxidative stress
  • bone marrow
  • pi k akt