A high-throughput and rapid method for accurate identification of emerging multidrug-resistant Candida auris.
Ausaf AhmadJonathan E SpencerShawn R LockhartSabrina SingletonDavid J PetwayDennis A BagarozziOwen T HerzeghPublished in: Mycoses (2019)
Candida auris is an emerging multidrug-resistant yeast associated with invasive infection in healthcare settings. Recently, C auris cases in the United States have been detected in 11 states with the majority of cases in New York, New Jersey and Illinois. Rapid and accurate identification of C auris is critical for patient care and the implementation of public health measures to control the spread of infection. Our aim was to develop and validate a rapid DNA extraction method using the Roche MagNA Pure 96 instrument and a TaqMan real-time PCR assay for reliable, high-throughput identification of C auris. We evaluated 247 patient dermal swab samples previously analysed by culture/MALDI-TOF. The diagnostic sensitivity and specificity were 93.6% and 97.2%, respectively. The assay was highly reproducible with a detection limit of 1 C auris CFU/10 μL. A receiver operating characteristic curve analysis of the real-time PCR data showed an area of 0.982 under the curve, with a CT cut-off value of ≤37.0. The turnaround time from DNA extraction to real-time PCR results was approximately 200 samples/day. In conclusion, we successfully validated a rapid and high-throughput method for accurate and reproducible identification of C auris with a significantly reduced turnaround time compared to culture/MALDI-TOF based methods.
Keyphrases
- real time pcr
- high throughput
- multidrug resistant
- mass spectrometry
- healthcare
- loop mediated isothermal amplification
- public health
- single cell
- high resolution
- gram negative
- primary care
- cell free
- drug resistant
- candida albicans
- magnetic resonance imaging
- acinetobacter baumannii
- case report
- klebsiella pneumoniae
- machine learning
- staphylococcus aureus
- cystic fibrosis
- sensitive detection
- social media
- patient reported outcomes
- health information
- affordable care act
- wound healing