Instant polarized light microscopy pi (IPOLπ) for quantitative imaging of collagen architecture and dynamics in ocular tissues.

Po-Yi LeeHannah SchilppNathan NaylorSimon C WatkinsBin Yang Ian A Sigal

Published in: bioRxiv : the preprint server for biology (2023)

We introduce IPOLπ, addressing IPOL limitations for characterizing eye collagen.IPOLπ orientation-encoded color cycle is 180° (π radians) instead of 90° in IPOL.IPOLπ requires a lower exposure time than IPOL, allowing faster imaging speed.IPOLπ visualizes non-birefringent tissues and backgrounds from brightness.IPOLπ is cheaper and less sensitive to imperfectly collimated light than IPOL.

Keyphrases

high resolution
gene expression
single molecule
high throughput
mass spectrometry
tissue engineering