Total Flavones of Rhododendron simsii Planch Flower Protect against Cerebral Ischemia-Reperfusion Injury via the Mechanism of Cystathionine-γ-Lyase-Produced H2S.
Shuo ChenJian-Hua ZhangYou-Yang HuDong-Hua HuShan-Shan GaoYi-Fei FanYu-Ling WangYi JiaoZhi-Wu ChenPublished in: Evidence-based complementary and alternative medicine : eCAM (2018)
Total flavones of Rhododendron simsii Planch flower (TFR) have a significant protective effect against cerebral ischemia-reperfusion injury. However, its mechanism is unclear. This study investigated the protection of TFR against cerebral ischemia-reperfusion injury via cystathionine-γ-lyase- (CSE-) produced H2S mechanism. CSE-/- mice and CSE-siRNA-transfected rat were used. Relaxation of cerebral basilar artery (CBA), H2S, and CSE mRNA were measured. TFR significantly inhibited cerebral ischemia-reperfusion-induced abnormal neurological symptom and cerebral infarct in the normal rats and the CSE+/+ mice, but not in the CSE-/- mice, and the inhibition was markedly attenuated in CSE-siRNA-transfected rat; TFR elicited a significant vasorelaxation in rat CBA, and the relaxation was markedly attenuated by removal of endothelium or CSE-siRNA transfection or coapplication of NO synthase inhibitor L-NAME and PGI2 synthase inhibitor Indo. CSE inhibitor PPG drastically inhibited TFR-evoked vasodilatation resistant to L-NAME and Indo in endothelium-intact rat CBA. TFR significantly increased CSE mRNA expression in rat CBA endothelial cells and H2S production in rat endothelium-intact CBA. The increase of H2S production resistant to L-NAME and Indo was abolished by PPG. Our data indicate that TFR has a protective effect against the cerebral ischemia-reperfusion injury via CSE-produced H2S and endothelial NO and/or PGI2 to relax the cerebral artery.
Keyphrases
- ischemia reperfusion injury
- subarachnoid hemorrhage
- oxidative stress
- cerebral ischemia
- endothelial cells
- nitric oxide
- brain injury
- type diabetes
- cerebral blood flow
- diabetic rats
- heart failure
- high fat diet induced
- acute myocardial infarction
- electronic health record
- coronary artery disease
- mass spectrometry
- blood brain barrier
- single molecule
- high resolution
- machine learning
- big data
- acute coronary syndrome
- percutaneous coronary intervention