Biologically synthesized silver nanoparticles as potent antibacterial effective against multidrug-resistant Pseudomonas aeruginosa.
C Campo-BeleñoR A Villamizar-GallardoL E López-JácomeE E GonzálezS Muñoz-CarranzaB FrancoR Morales-EspinosaR Coria-JimenezR Franco-CendejasM Hernández-DuránReyna Lara-MartínezL F Jiménez-GarcíaA M Fernández-PresasRodolfo Garcia-ContrerasPublished in: Letters in applied microbiology (2022)
Pseudomonas aeruginosa is one of the most worrisome infectious bacteria due to its intrinsic and acquired resistance against several antibiotics and the recalcitrance of its infections; hence, the development of novel antimicrobials effective against multidrug-resistant P. aeruginosa is mandatory. In this work, silver nanoparticles obtained by green synthesis using a leaf extract and fungi were tested against a battery of clinical strains from cystic fibrosis, pneumonia and burnt patients, some of them with multidrug resistance. Both nanoparticles showed a potent antibacterial effect, causing severe damage to the cell wall, membrane and DNA, and inducing the production of reactive oxygen species. Moreover, the nanoparticles derived from fungi showed synergistic antibacterial effects with the antibiotics meropenem and levofloxacin for some clinical strains and both kinds of nanoparticles were nontoxic for larvae of the moth Galleria mellonella, encouraging further research for their implementation in the treatment of P. aeruginosa infections.
Keyphrases
- silver nanoparticles
- pseudomonas aeruginosa
- multidrug resistant
- cystic fibrosis
- acinetobacter baumannii
- gram negative
- cell wall
- reactive oxygen species
- escherichia coli
- drug resistant
- end stage renal disease
- biofilm formation
- newly diagnosed
- ejection fraction
- healthcare
- prognostic factors
- lung function
- peritoneal dialysis
- oxidative stress
- circulating tumor
- early onset
- cancer therapy
- walled carbon nanotubes
- zika virus
- intensive care unit
- drug delivery
- aedes aegypti
- respiratory failure
- air pollution
- replacement therapy