Two-Photon Probes for Golgi Apparatus: Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy.
Ji-Woo ChoiSeung Taek HongMun Seok KimKyu Cheol PaikMan So HanBong Rae ChoPublished in: Analytical chemistry (2019)
We have developed blue- and yellow-emitting two-photon probes (BGolgi-blue and PGolgi-yellow) from 6-(benzo[ d]oxazol-2-yl)-2-naphthalylamine and 2,5-bis(benzo[ d]oxazol-2-yl)pyrazine derivatives as the fluorophores and trans-Golgi-network peptide (SDYQRL) as the Golgi-apparatus-targeting moiety. HeLa cells labeled with BGolgi-blue and PGolgi-yellow emitted two-photon-excited fluorescence at 462 and 560 nm, respectively, with effective two-photon-action cross-section values of 1860 and 1600 × 10-50 cm4·s/photon, respectively. The probes can detect the Golgi apparatus in live cells and deep inside live tissue via two-photon microscopy at widely separated wavelength regions with high selectivity and minimal pH interference, and they are photostable and have low cytotoxicity.
Keyphrases
- living cells
- single molecule
- fluorescent probe
- induced apoptosis
- endoplasmic reticulum
- cell cycle arrest
- small molecule
- monte carlo
- light emitting
- label free
- cell death
- nucleic acid
- photodynamic therapy
- computed tomography
- endoplasmic reticulum stress
- quantum dots
- energy transfer
- oxidative stress
- high speed
- pet imaging
- sensitive detection
- network analysis
- structural basis