Anti-inflammatory Potential of Saponins from Aster tataricus via NF-κB/MAPK Activation.
Xiang-Dong SuHyun-Jae JangCai-Yi WangSeung Woong LeeMun-Chual RhoNguyen Phuong ThaoSeo Young YangPublished in: Journal of natural products (2019)
Four new aster saponins (1-4) together with five known analogues (5-9) were isolated from Aster tataricus. The chemical structures of 1-4 were elucidated based on spectrometric and spectroscopic analysis and comparison with reported data. The potential anti-inflammatory activities of aster saponins 1-9 were evaluated subsequently by measuring lipopolysaccharide (LPS)-enhanced nitric oxide (NO) formation in murine macrophages. Among these, aster saponin B (6) exhibited the most potent inhibitory activity (IC50: 1.2 μM). Additionally, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein levels were dose-dependently suppressed by 6 in LPS-activated RAW 264.7 cells. Investigation of the anti-inflammatory mechanism indicated that 6 attenuated the phosphorylation and degradation of the inhibitor of NF-κB (IκB), which led to the blocking of NF-κB p65 translocation to the nucleus.
Keyphrases
- anti inflammatory
- nitric oxide synthase
- nitric oxide
- signaling pathway
- lps induced
- pi k akt
- induced apoptosis
- oxidative stress
- inflammatory response
- cell cycle arrest
- nuclear factor
- molecular docking
- hydrogen peroxide
- toll like receptor
- electronic health record
- high resolution
- immune response
- risk assessment
- big data
- binding protein
- amino acid
- small molecule
- high performance liquid chromatography
- molecular dynamics simulations
- deep learning
- ms ms
- cell death
- artificial intelligence