Thyroid-stimulating hormone receptor signaling restores skeletal muscle stem cell regeneration in rats with muscular dystrophy.
Valentina TagliettiKaouthar KefiLea RiveraOriane BergiersNastasia CardoneFanny CoulpierStamatia GioftsidiBernadette Drayton-LibotteCyrielle HouFrançois Jérôme AuthierPietri-Rouxel FranceMatthieu P RobertDominique Bremond-GignacClaudio BrunoChiara FiorilloEdoardo MalfattiPeggy LafusteLaurent TiretFrederic RelaixPublished in: Science translational medicine (2023)
Duchenne muscular dystrophy (DMD) is a severe and progressive myopathy leading to motor and cardiorespiratory impairment. We analyzed samples from patients with DMD and a preclinical rat model of severe DMD and determined that compromised repair capacity of muscle stem cells in DMD is associated with early and progressive muscle stem cell senescence. We also found that extraocular muscles (EOMs), which are spared by the disease in patients, contain muscle stem cells with long-lasting regenerative potential. Using single-cell transcriptomics analysis of muscles from a rat model of DMD, we identified the gene encoding thyroid-stimulating hormone receptor ( Tshr ) as highly expressed in EOM stem cells. Further, TSHR activity was involved in preventing senescence. Forskolin, which activates signaling downstream of TSHR, was found to reduce senescence of skeletal muscle stem cells, increase stem cell regenerative potential, and promote myogenesis, thereby improving muscle function in DMD rats. These findings indicate that stimulation of adenylyl cyclase leads to muscle repair in DMD, potentially providing a therapeutic approach for patients with the disease.
Keyphrases
- stem cells
- duchenne muscular dystrophy
- skeletal muscle
- muscular dystrophy
- single cell
- insulin resistance
- cell therapy
- dna damage
- multiple sclerosis
- endothelial cells
- newly diagnosed
- rna seq
- early onset
- spinal cord injury
- neuropathic pain
- gene expression
- type diabetes
- spinal cord
- prognostic factors
- oxidative stress
- late onset
- genome wide
- bone marrow
- peritoneal dialysis
- genome wide identification