Grp78 Loss in Epithelial Progenitors Reveals an Age-linked Role for Endoplasmic Reticulum Stress in Pulmonary Fibrosis.
Zea BorokMasafumi HoriePer FlodbyHongjun WangYixin LiuSivagini GaneshAmy L FirthParviz MinooChanggong LiMichael F BeersAmy S LeeBeiyun ZhouPublished in: American journal of respiratory and critical care medicine (2020)
Rationale: Alveolar epithelial cell (AEC) injury and dysregulated repair are implicated in the pathogenesis of pulmonary fibrosis. Endoplasmic reticulum (ER) stress in AEC has been observed in idiopathic pulmonary fibrosis (IPF), a disease of aging.Objectives: To investigate a causal role for ER stress in the pathogenesis of pulmonary fibrosis (PF) and therapeutic potential of ER stress inhibition in PF.Methods: The role of ER stress in AEC dysfunction and fibrosis was studied in mice with tamoxifen (Tmx)-inducible deletion of ER chaperone Grp78, a key regulator of ER homeostasis, in alveolar type II (AT2) cells, progenitors of distal lung epithelium, and in IPF lung slice cultures.Measurements and Main Results: Grp78 deletion caused weight loss, mortality, lung inflammation, and spatially heterogeneous fibrosis characterized by fibroblastic foci, hyperplastic AT2 cells, and increased susceptibility of old and male mice, all features of IPF. Fibrosis was more persistent in more severely injured Grp78 knockout (KO) mice. Grp78 KO AT2 cells showed evidence of ER stress, apoptosis, senescence, impaired progenitor capacity, and activation of TGF-β (transforming growth factor-β)/SMAD signaling. Glucose-regulated protein 78 is reduced in AT2 cells from old mice and patients with IPF, and ER stress inhibitor tauroursodeoxycholic acid ameliorates ER stress and fibrosis in Grp78 KO mouse and IPF lung slice cultures.Conclusions: These results support a causal role for ER stress and resulting epithelial dysfunction in PF and suggest ER stress as a potential mechanism linking aging to IPF. Modulation of ER stress and chaperone function may offer a promising therapeutic approach for pulmonary fibrosis.
Keyphrases
- endoplasmic reticulum stress
- induced apoptosis
- idiopathic pulmonary fibrosis
- pulmonary fibrosis
- endoplasmic reticulum
- transforming growth factor
- oxidative stress
- cell cycle arrest
- interstitial lung disease
- epithelial mesenchymal transition
- weight loss
- cell death
- blood pressure
- coronary artery disease
- estrogen receptor
- computed tomography
- dna damage
- risk factors
- magnetic resonance imaging
- clinical trial
- body mass index
- cardiovascular disease
- mouse model
- signaling pathway
- small molecule
- risk assessment
- heat shock
- binding protein
- cell fate
- protein protein
- climate change