Molecular Analysis with16s rRNA PCR/Sanger Sequencing and Molecular Antibiogram performed on DNA extracted from valve improve diagnosis and targeted therapy of Infective Endocarditis: a prospective study.
Alessandra MularoniMalgorzata MikulskaFloriana BarberaElena GrazianoAlice Annalisa MedagliaDaniele Di CarloFrancesco MonacoDiego BellaviaAntonio CascioGiuseppe RaffaSergio SciaccaAngelo LucaMichele PilatoPier Giulio ConaldiPublished in: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America (2022)
MA showed a high specificity and sensitivity in etiological diagnosis of IE. Molecular antibiogram could overcome the major limitation of MA that is the lack of susceptibility testing. We advocate for the inclusion of MA among diagnostic criteria for IE and for a more extensive use of molecular antibiogram when the culture result is negative and MA is the only positive test.