Effects of calcium concentration on nonviral gene delivery to bone marrow-derived stem cells.

Timothy M AcriNoah Z LairdSean M GearyAliasger K Salem Kyungsup Shin

Published in: Journal of tissue engineering and regenerative medicine (2019)

Ca2+ at a 10 mM concentration can significantly reduce toxicity and enhance transfection efficiency when combined with PEI-pDNA complexes, and this combination can be specifically applied to further enhance the differentiation of BMSCs by using the combination of polyethyleneimine-plasmid bone morphogenetic protein 2 (PEI-pBMP-2) and 10 mM Ca2+ as compared with PEI-pBMP-2 alone.

Keyphrases

stem cells
escherichia coli
oxidative stress
mesenchymal stem cells
protein kinase
crispr cas
cell therapy
bone marrow