Diagnostic Performance of Anti-dsDNA Tests by Indirect Immunofluorescence and Enzyme-linked Immunosorbent Assay in Patients with Systemic Lupus Erythematosus.
Ivana Nieto-AristizábalLaura Jackeline EspañaJorge OrtegaIngrid Ruiz-OrdoñezÁlvaro J VivasGloria L CastañoCarmen CastilloMario Bautista-VargasAna SuárezCarlos A CañasGabriel J TobónPublished in: The journal of applied laboratory medicine (2023)
Anti-dsDNA detection by IIF and EIA are complementary and may indicate different clinical patterns in patients with SLE. The detection of anti-dsDNA antibodies by both techniques has a higher PPV than either separately for confirming SLE diagnosis or flares. These results highlight the need for evaluating both methods in clinical practice.