Bromopyrylium Derivatization Facilitates Identification by Mass Spectrometry Imaging of Monoamine Neurotransmitters and Small Molecule Neuroactive Compounds.
Reza ShariatgorjiAnna NilssonNicole StrittmatterTheodosia VallianatouXiaoqun ZhangPer SvenningssonRichard J A GoodwinPer E AndrenPublished in: Journal of the American Society for Mass Spectrometry (2020)
Mass spectrometry imaging using matrix-assisted laser desorption/ionization and desorption electrospray ionization has recently been employed to investigate the distribution of neurotransmitters, including biogenic amines and amino acids, directly in brain tissue sections. Ionization is facilitated by charge-tagging through pyrylium derivatization of primary amine containing neurotransmitters directly in tissue sections, significantly improving the limit of detection. Since the derivatization adds carbon and hydrogen to the target compounds, the resulting isotopic patterns of the products are not distinctive from those of the nonderivatized species. Here, we describe an approach for chemically modifying the reactive pyrylium ion to introduce the distinct isotopic signature of bromine in mass spectra of chemically derivatized substances in tissue sections. The method enables monoamine compounds to be distinguished directly in tissue sections, facilitating their identification.
Keyphrases
- mass spectrometry
- gas chromatography
- liquid chromatography
- high performance liquid chromatography
- small molecule
- high resolution
- ms ms
- tandem mass spectrometry
- liquid chromatography tandem mass spectrometry
- gas chromatography mass spectrometry
- simultaneous determination
- amino acid
- high resolution mass spectrometry
- solid phase extraction
- drinking water
- multiple sclerosis
- brain injury
- blood brain barrier
- subarachnoid hemorrhage
- photodynamic therapy
- cerebral ischemia
- genetic diversity
- label free
- density functional theory