Trametes versicolor Laccase-Based Magnetic Inorganic-Protein Hybrid Nanobiocatalyst for Efficient Decolorization of Dyes in the Presence of Inhibitors.

In the present investigation, an ecofriendly magnetic inorganic-protein hybrid system-based enzyme immobilization was developed using partially purified laccase from Trametes versicolor ( Tv Lac), Fe 3 O 4 nanoparticles, and manganese (Mn), and was successfully applied for synthetic dye decolorization in the presence of enzyme inhibitors. After the partial purification of crude Tv Lac, the specific enzyme activity reached 212 U∙mg total protein -1 . The synthesized Fe 3 O 4 /Mn 3 (PO 4 ) 2 -laccase (Fe 3 O 4 /Mn- Tv Lac) and Mn 3 (PO 4 ) 2 -laccase (Mn- Tv Lac) nanoflowers (NFs) exhibited encapsulation yields of 85.5% and 90.3%, respectively, with relative activities of 245% and 260%, respectively, compared with those of free Tv Lac. One-pot synthesized Fe 3 O 4 /Mn- Tv Lac exhibited significant improvements in catalytic properties and stability compared to those of the free enzyme. Fe 3 O 4 /Mn- Tv Lac retained a significantly higher residual activity of 96.8% over that of Mn- Tv Lac (47.1%) after 10 reuse cycles. The NFs showed potential for the efficient decolorization of synthetic dyes in the presence of enzyme inhibitors. For up to five reuse cycles, Fe 3 O 4 /Mn- Tv Lac retained a decolorization potential of 81.1% and 86.3% for Coomassie Brilliant Blue R-250 and xylene cyanol, respectively. The synthesized Fe 3 O 4 /Mn- Tv Lac showed a lower acute toxicity towards Vibrio fischeri than pure Fe 3 O 4 nanoparticles did. This is the first report of the one-pot synthesis of biofriendly magnetic protein-inorganic hybrids using partially purified Tv Lac and Mn.