An ultrasensitive electrochemical biosensor for microRNA-21 detection via AuNPs/GAs and Y-shaped DNA dual-signal amplification strategy.
Congjuan HeJiaying ZhaoYanyi LongHuisi YangJiangbo DongHuan LiuZhikun HuMei YangDanqun HuoChangjun HouPublished in: Chemical communications (Cambridge, England) (2022)
Herein, a gold nanoparticles/graphene aerogels (AuNPs/GAs) modified electrochemical biosensor with catalytic hairpin assembly (CHA) and Y-shaped DNA nanostructure dual-signal amplification approaches for ultrasensitive microRNA-21 (miR-21) detection was successfully constructed, which displayed an ultra-wide detection linear range from 5 fM to 50 nM, as well as a relatively low detection limit (LOD) of 14.70 aM (S/N = 3). Furthermore, the sensing strategy had excellent specificity among highly homologous miRNA family members and exhibited satisfactory analytical performance for miRNA detection.
Keyphrases
- label free
- gold nanoparticles
- loop mediated isothermal amplification
- real time pcr
- quantum dots
- cell proliferation
- room temperature
- nucleic acid
- reduced graphene oxide
- dna damage
- long non coding rna
- sensitive detection
- circulating tumor
- high resolution
- mass spectrometry
- photodynamic therapy
- molecularly imprinted
- long noncoding rna
- carbon dioxide
- liquid chromatography
- electron transfer
- circulating tumor cells