Dual-procedural separation of CTCs in cutaneous melanoma provides useful information for both molecular diagnosis and prognosis.
Marco TucciStella D'OronzoFrancesco MannavolaClaudia FeliciDomenica LoveroPaola CafforioRaffaele PalmirottaFranco SilvestrisPublished in: Therapeutic advances in medical oncology (2020)
The dual-step protocol of immune-magnetic sorting and subsequent dielectrophoretic DEPArray separation, turned out to be a suitable method to isolate viable CTCs from stage IV melanoma patients and enabled quantitative and qualitative analyses on these cells, which may deserve prospective evaluation for potential use in the clinical practice.
Keyphrases
- circulating tumor cells
- clinical practice
- end stage renal disease
- induced apoptosis
- newly diagnosed
- ejection fraction
- chronic kidney disease
- liquid chromatography
- prognostic factors
- high resolution
- skin cancer
- signaling pathway
- mass spectrometry
- oxidative stress
- endoplasmic reticulum stress
- social media
- basal cell carcinoma