Ratiometric optical detection of pyrophosphate based on aggregation-caused dual-signal response of gold nanoclusters.
Hui-Ting CaoTiansheng ZhaoMeng-Qian LiuLi-Zhen GuoYong-Qin HeKun ZhangXi MaiNa LiPublished in: Luminescence : the journal of biological and chemical luminescence (2023)
Sensing of pyrophosphate ion (PPi) has received much attention due to the strong demand for clinical diagnostics. Herein, based on gold nanoclusters (Au NCs), a ratiometric optical detection method for PPi is developed by simultaneously detecting the dual-signals of fluorescence (FL) and second-order scattering (SOS). The PPi is detected by inhibiting the formation of aggregates of Fe 3+ with Au NCs. Binding of Fe 3+ to Au NCs causes aggregation of Au NCs, which leads to fluorescence quenching and scattering increasing. The presence of PPi can competitively bind Fe 3+ to re-disperse the Au NCs and finally recover the fluorescence and reduce the scattering signal. The designed PPi sensor shows a high sensitivity with a linear range of 5-50 μM and a detection limit of 1.2 μM. In addition, the assay has excellent selectivity for PPi, which makes its application in real biological samples extremely valuable.
Keyphrases
- sensitive detection
- loop mediated isothermal amplification
- protein protein
- energy transfer
- quantum dots
- label free
- small molecule
- single molecule
- reduced graphene oxide
- real time pcr
- visible light
- high resolution
- high speed
- high throughput
- working memory
- hydrogen peroxide
- nitric oxide
- single cell
- aqueous solution
- signaling pathway
- structural basis