Changes of synaptic vesicles in three-dimensional synapse models by treatment with umbelliferone in scopolamine-induced hippocampal injury model.
Ga-Young ChoiEunyoung MoonHyosung ChoiHee-Seok KweonPublished in: Applied microscopy (2024)
The neuroprotective effects of umbelliferone (UMB) were visualized in three-dimensional (3D) images on vesicle density changes of organotypic hippocampal slice tissues (OHSCs) induced by scopolamine by high voltage electron microscopy. Observations revealed that the number of vesicles decreased in OHSCs induced by scopolamine, and UMB was found to inhibit scopolamine-induced reduction in vesicles, resulting in an increase in vesicle count. These 3D models provide valuable insight for understanding the increase of synapse vesicles in hippocampal tissues treated with UMB.
Keyphrases
- cerebral ischemia
- high glucose
- electron microscopy
- diabetic rats
- gene expression
- deep learning
- drug induced
- temporal lobe epilepsy
- magnetic resonance imaging
- oxidative stress
- brain injury
- computed tomography
- subarachnoid hemorrhage
- optical coherence tomography
- convolutional neural network
- combination therapy
- image quality