Aggregation-Induced Emission Probes for Specific Turn-On Quantification of Bovine Serum Albumin.
Yuan YuQing-Tian GongWei-Fan LuYan-Hong LiuZeng-Jie YangNa WangXiao-Qi YuPublished in: ACS applied bio materials (2020)
A series of aggregation-induced emission (AIE) fluorescence probes, coined 4H-pyrimido[2,1- b ]benzothiazole derivatives, has been synthesized by Biginelli reactions. Utilizing spectroscopic techniques, their photophysical properties have been comprehensively investigated in working environment both in vitro and in vivo . Density functional theory (DFT) calculations were performed to better understand the mechanism of these probes. The interactions between 4H-pyrimido[2,1- b ]benzothiazoles with different substituents and bovine serum albumin (BSA) were analyzed using UV-vis and fluorescence spectroscopy, synchronous fluorescence, and docking analysis. Studies found that 4H-pyrimido[2,1- b ]benzothiazole could bind to bovine serum albumin (BSA) through a hydrogen bond and hydrophobic interactions, resulting in enhancement of fluorescence emission of probes 1a - 6f and fluorescence quenching of BSA. Combined with cell imaging experiments, these provide information on potential effects of benzothiazoles on disease treatment.
Keyphrases
- single molecule
- living cells
- density functional theory
- molecular dynamics
- energy transfer
- atomic force microscopy
- small molecule
- fluorescence imaging
- fluorescent probe
- high resolution
- molecular docking
- molecular dynamics simulations
- single cell
- healthcare
- solid state
- risk assessment
- mesenchymal stem cells
- quantum dots
- health information
- photodynamic therapy
- human health
- visible light