Highly sensitive quantification of site-specific 5-hydroxymethylcytosine at single-base resolution by HpaII-mediated ligation PCR.

Zhenhao ZhangXiao ShanPengbo ZhangWeiliang LiuJingli YanZhengping Li

Published in: Organic & biomolecular chemistry (2019)

A highly sensitive HpaII-mediated ligation PCR assay that can precisely discriminate 5hmC from 5mC and C is developed for the quantitative determination of site-specific 5hmC in genomic DNA samples.

Keyphrases

molecularly imprinted
single molecule
fluorescent probe
living cells
high throughput
circulating tumor
copy number
mass spectrometry
dna methylation
simultaneous determination