Analysis of Electric Field Stimulation in Blue Light Stressed 661W Cells.
Sharanya BolaPallavi SubramanianDaniela CalziaAndreas DahlIsabella PanfoliRichard H W FunkCora RoehleckePublished in: International journal of molecular sciences (2023)
Though electrical stimulation is used as a therapeutic approach to treat retinal and spinal injuries, many protective mechanisms at cellular level have not been elucidated. We performed a detailed analysis of cellular events in blue light (Li) stressed 661W cells, which were subjected to direct current electric field (EF) stimulation. Our findings revealed that EF stimulation induced protective effects in 661W cells from Li-induced stress by multiple defense mechanisms, such as increase in mitochondrial activity, gain in mitochondrial potential, increase in superoxide levels, and the activation of unfolded protein response (UPR) pathways, all leading to an enhanced cell viability and decreased DNA damage. Here, our genetic screen results revealed the UPR pathway to be a promising target to ameliorate Li-induced stress by EF stimulation. Thus, our study is important for a knowledgeable transfer of EF stimulation into clinical application.
Keyphrases
- oxidative stress
- diabetic rats
- induced apoptosis
- high glucose
- dna damage
- cell cycle arrest
- endoplasmic reticulum stress
- drug induced
- single cell
- spinal cord injury
- cell death
- endothelial cells
- high throughput
- gene expression
- genome wide
- diabetic retinopathy
- hydrogen peroxide
- solid state
- heat stress
- protein protein
- climate change