Exploring the antimicrobial efficacy of Manuka honey against multidrug-resistant and extensively drug-resistant Salmonella Typhi causing septicemia in Pakistan.
Iqra BashirMuhammad Hidayat RasoolMuhammad ShafiqueKokab JabeenMuhammad Usman QamarPublished in: Future microbiology (2024)
Aim: To determine the efficacy of manuka honey against multidrug-resistant (MDR) and extensively drug-resistant (XDR) clinical strains of Salmonella Typhi. Materials & methods: Clinical isolates were processed using the Bactec blood culture system, identification and antibiogram by Vitek 2 and antibiotic resistance genes through polymerase chain reaction (PCR). Microbroth dilution assays evaluated the antibacterial activity of manuka honey. Results: MDR and XDR- S. Typhi was susceptible to azithromycin. These strains carried the H58, gyrA , gyrB , bla CTX-M-15 , and bla TEM-1 genes. At 100% honey, the zone of inhibition for MDR (15-23 mm) and XDR (15-24 mm) strains. 18/50 MDR and 14/50 XDR strains inhibited at 3.125 v/v% killed at 6.25 v/v% concentration respectively. Conclusion: Manuka honey could be an alternative option for treating S. Typhi infections.
Keyphrases
- multidrug resistant
- drug resistant
- klebsiella pneumoniae
- escherichia coli
- acinetobacter baumannii
- gram negative
- antibiotic resistance genes
- wastewater treatment
- staphylococcus aureus
- genome wide
- listeria monocytogenes
- bioinformatics analysis
- mass spectrometry
- silver nanoparticles
- anaerobic digestion
- liquid chromatography
- high resolution
- transcription factor
- simultaneous determination