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Design of the elusive proteinaceous oxygen donor copper site suggests a promising future for copper for MRI contrast agents.

Anokhi ShahMichael J TaylorGiulia MolinaroSellamuthu AnbuMargaux VerduLucy JenningsIuliia MikulskaM Concepción SánchezHassane El MkamiGraham M SmithMelanie M BrittonJanet Eleanor LovettAnna F A Peacock
Published in: Proceedings of the National Academy of Sciences of the United States of America (2023)
We report the preparation and spectroscopic characterization of a highly elusive copper site bound exclusively to oxygen donor atoms within a protein scaffold. Despite copper generally being considered unsuitable for use in MRI contrast agents, which in the clinic are largely Gd(III) based, the designed copper coiled coil displays relaxivity values equal to, or superior than, those of the Gd(III) analog at clinical field strengths. The creation of this new-to-biology proteinaceous CuO x -binding site demonstrates the power of the de novo peptide design approach to access chemistry for abiological applications, such as for the development of MRI contrast agents.
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