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Measuring Rosetting Inhibition in Plasmodium falciparum Parasites Using a Flow Cytometry-Based Assay.

Maria Del Pilar QuintanaJun-Hong Ch'ng
Published in: Methods in molecular biology (Clifton, N.J.) (2022)
Rosetting is the ability of Plasmodium falciparum-infected erythrocytes (IEs) to bind to host receptors on the surface of uninfected erythrocytes (uE) leading to the formation of a cluster of cells with a central IE surrounded by uE. It is a hallmark event during the pathogenesis of P. falciparum malaria, the most severe species causing malaria, which affects mostly young children in Africa. There are no current treatments effectively targeting and disrupting parasite rosette formation. Here, we detail a high-throughput, flow cytometry based assay that allows testing and identification of potential rosetting-inhibitory compounds that could be used in combination with anti-plasmodial drugs to reduce malaria morbidity and mortality.
Keyphrases
  • plasmodium falciparum
  • flow cytometry
  • high throughput
  • induced apoptosis
  • single cell
  • hiv infected
  • early onset
  • endoplasmic reticulum stress
  • risk assessment
  • drug delivery