Microbial synthesis of Prussian blue for potentiating checkpoint blockade immunotherapy.
Dongdong WangJiawei LiuChanglai WangWeiyun ZhangGuangbao YangYun ChenXiaodong ZhangYinglong WuLong GuHongzhong ChenWei YuanXiaokai ChenGuofeng LiuBin GaoQianwang ChenYanli ZhaoPublished in: Nature communications (2023)
Cancer immunotherapy is revolutionizing oncology. The marriage of nanotechnology and immunotherapy offers a great opportunity to amplify antitumor immune response in a safe and effective manner. Here, electrochemically active Shewanella oneidensis MR-1 can be applied to produce FDA-approved Prussian blue nanoparticles on a large-scale. We present a mitochondria-targeting nanoplatform, MiBaMc, which consists of Prussian blue decorated bacteria membrane fragments having further modifications with chlorin e6 and triphenylphosphine. We find that MiBaMc specifically targets mitochondria and induces amplified photo-damages and immunogenic cell death of tumor cells under light irradiation. The released tumor antigens subsequently promote the maturation of dendritic cells in tumor-draining lymph nodes, eliciting T cell-mediated immune response. In two tumor-bearing mouse models using female mice, MiBaMc triggered phototherapy synergizes with anti-PDL1 blocking antibody for enhanced tumor inhibition. Collectively, the present study demonstrates biological precipitation synthetic strategy of targeted nanoparticles holds great potential for the preparation of microbial membrane-based nanoplatforms to boost antitumor immunity.
Keyphrases
- immune response
- dendritic cells
- cell death
- lymph node
- cancer therapy
- microbial community
- photodynamic therapy
- mouse model
- type diabetes
- reactive oxygen species
- toll like receptor
- palliative care
- risk assessment
- cell proliferation
- regulatory t cells
- oxidative stress
- magnetic resonance imaging
- signaling pathway
- quantum dots
- magnetic resonance
- drug delivery
- computed tomography
- drug release