An indole-based fluorescent chemosensor targeting the autophagosome.
Sun Young ParkKyutae KimDong-Hyung ChoEun-Young JoChulhun KangMin Hee LeePublished in: Chemical communications (Cambridge, England) (2022)
Autophagy is a process for the degradation and recycling of intracellular components and dysfunctional organelles. We developed an indole-embedded fluorescent naphthalimide for the selective imaging of autophagosomes in live cells. It was shown as intense puncta in the fluorescence confocal images and co-localizes with an autophagosome marker, LC3-RFP. In addition, it was applied to cellular autophagic models based on ER stress and starvation to verify its capability.
Keyphrases
- cell death
- cell cycle arrest
- induced apoptosis
- quantum dots
- living cells
- endoplasmic reticulum stress
- optical coherence tomography
- fluorescent probe
- high resolution
- signaling pathway
- oxidative stress
- deep learning
- single molecule
- label free
- convolutional neural network
- simultaneous determination
- cancer therapy
- energy transfer
- mass spectrometry
- solid phase extraction