High-Resolution Melting (HRM) analysis of DNA methylation using semiconductor chip-based digital PCR.

Jinuk JeongYongsu YangMin-Sik SongHee-Young WonAndrew T HanSongmi Kim

Published in: Genes & genomics (2024)

The present study shows that LOAA dPCR could detect different melting temperatures according to methylation status of target sequences, indicating that LOAA dPCR would be useful for diagnostic applications that require the accurate quantification and assessment of DNA methylation.

Keyphrases

high resolution
dna methylation
genome wide
gene expression
mass spectrometry
high speed
tandem mass spectrometry
copy number
room temperature
clinical evaluation