Top-Down Proteomics Identifies Plasma Proteoform Signatures of Liver Cirrhosis Progression.

Eleonora ForteJes M SandersIndira PlaVijaya Lakshmi KanchustambhamMichael A R HollasChe-Fan HuangAniel SanchezKatrina N PetersonRafael D MelaniAlexander HuangPraneet PolineniJulianna M DollZachary DietchMichael P SnyderDaniela P Ladner

Published in: bioRxiv : the preprint server for biology (2024)

Performed a pilot top-down LC-MS/MS analysis to identify proteoforms (PFRs) in the plasma of patients with 3 progressive stages of liver cirrhosis.Identified 2867 proteoforms (PFRs) and 209 differentially regulated proteoforms (DRPs) in the different stages of the disease.Identified DRP profiles able to potentially distinguish early from late stages of the disease, including 115 liver-derived DRPs.Fibrinogen alpha chain, haptoglobin, and Apo A-I are the proteins with the highest number of DRPs and represent potential candidate biomarkers of liver cirrhosis progression.

Keyphrases

genome wide
multiple sclerosis
mass spectrometry
transcription factor
risk assessment
dna methylation