Substrate-Induced Growth of Micro/Nanostructured Zn(OH)F Arrays for Highly Sensitive Microfluidic Fluorescence Assays.
De ZhaoZhihua WuWei ZhangJian YuHe LiWen DiYourong DuanPublished in: ACS applied materials & interfaces (2021)
To date, ZnO array-based microfluidic fluorescence assays have been widely investigated and have exhibited excellent performance in the detection of cancer biomarkers. However, the requirements of highly sensitive detection necessitate further improvement of current Zn-based fluorescence detection devices. Here, a rhombus-like Zn(OH)F array-based microfluidic fluorescence detection device is proposed. Construction of Zn(OH)F arrays on the inner wall of a microchannel is carried out via a microfluidic chemical method. A substrate-induced growth strategy for Zn(OH)F arrays is proposed, and various micro/nanostructured Zn(OH)F arrays are successfully obtained. Zn(OH)F nanorod arrays with a high aspect ratio can be constructed on the columnar ZnO nanorod arrays, and the results indicate that the fluorescence enhancement factor (EF) of the Zn(OH)F arrays toward Cy3 is approximately 4-fold that of the ZnO nanorod arrays, which can be attributed to the higher excitation light absorption and evanescent electric field. In human epididymis-specific protein 4 (HE4) detection, the limit of detection (LOD) reaches 9.3 fM, and the dynamic linear range is 10 fM to 100 pM. It has been demonstrated that Zn(OH)F nanorod array-based microfluidic devices are excellent fluorescence assay platforms that also provide a new design and construction strategy for fluorescence enhancement substrates for the detection of biomarkers.
Keyphrases
- high throughput
- label free
- heavy metals
- loop mediated isothermal amplification
- high density
- single molecule
- energy transfer
- sensitive detection
- real time pcr
- single cell
- quantum dots
- circulating tumor cells
- endothelial cells
- high glucose
- air pollution
- room temperature
- drug induced
- mass spectrometry
- particulate matter
- reduced graphene oxide
- protein protein
- atomic force microscopy
- liquid chromatography
- stress induced
- childhood cancer