To assess the performance of adaptive optics and predict an optimal wavefront correction, we built a wavefront reconstructor with a damped transpose matrix of the influence function. Using an integral control strategy, we tested this reconstructor with four deformable mirrors in an experimental system, an adaptive optics scanning laser ophthalmoscope, and an adaptive optics near-confocal ophthalmoscope. Testing results proved that this reconstructor could ensure a stable and precise correction for wavefront aberration compared to a conventional optimal reconstructor formed by the inverse matrix of the influence function. This method may provide a helpful tool for testing, evaluating, and optimizing adaptive optics systems.